Co - Mentor
Cellular models of seizure related disorders.
Drs. Murphy and Parent have a long standing collaboration centered on understanding the neural basis of epilepsy. Previous work has focused on the link between temporal lobe epilepsy and adult born neurons in the dentate gyrus (PMID 25429123, PMID 31270158). Currently the Murphy/Parent labs are collaborating on studies of 1) genetic epilepsy mechanisms using patient-derived or gene edited induced pluripotent stem cell-derived neurons, cultured in 2-D and as brain organoids; and 2) preclinical epilepsy therapy studies using human pluripotent stem cell-derived interneuron progenitor transplantation into rodent epilepsy models. The in vitro work will involve calcium imaging, extracellular recordings in whole organoids and multielectrode array recordings to assess epilepsy-like phenotypes. The transplantation studies include EEG monitoring, behavioral assays and in vitro rodent brain slice recordings.